Comparison of graft survival between full thickness and lamellar pig-to-monkey corneal xenotransplantation from the same genetically engineered pig with minimal immunosuppression
Ik Jin Yun1, Ki Cheul Shin2, Wan Seop Kim3, Keon Bong Oh4, Hye Sun Shin1, Yu Rim Ahn1.
1Surgery, Konkuk University Hospital, Seoul, Korea; 2Ophthalmology, Konkuk University Hospital, Seoul, Korea; 3Pathology, Konkuk University Hospital, Seoul, Korea; 4Animal Biotechnology, National Instutute of Animal Science, Jeonju, Korea
Introduction: Graft survival of full thickness corneal xenotransplantation with minimal immunosuppression in genetically engineered pig is not well known. In comparison, lamella corneal xenotransplantation showed good results. We compare the graft survival between full thickness and lamella with the same genetically engineered pig.
Method: With the use of 3 transgenic pigs, six pig-to-monkey corneal transplantation was done. Two corneas from one pig transpanted to two monkey with the fullthickness and lamella corneal xenotransplantations each. Transgenic type of donor pig is GTKO+CD46 in one and GTKO+CD46+TBM in two.
Results: Graft survivals of each xenotransplantation of GTKO+CD 46 are 28 days and 28 days same. With the add of TBM, survival differences between lamella and fullthickness is 98 vs. 14 days and 119+(on-going) vs. 21 days. For failed graft, many inflammatory cells exist in grafts and no inflammatory cell in recipient's stromal bed.
Conclusions: Lamella xenocorneal transplantation has the advantage of not having surgical complication such as retrocorneal membrane or anterior synechia seen in full thickness corneal xenotransplantation. In our study, graft survival of lamellar xenotransplantation is not well comparing with previous our experiments although superior survival period to fullthickness. Difference of graft survival based on the transpenic type is not definitive also. More cases and improvement of graft survival of lammella xenotransplantation will be needed to check the possibility of full thickness coreneal xenotransplantation with the use of transgenic pig and minimal immunosuppression.
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