Bionic pancteas – 3D bioprinting of a bionic organ with a vascular system – results of transplantation in large animals
Michal Wszola1,2, Andrzej Berman1, Marta Klak1,2, Tomasz Dobrzanski1, Marta Kolodzijska2, Tomasz Bryniarski2, Jaroslaw Wejman3, Dominika Szkopek4, Katarzyna Roszkowicz-Ostrowska4, Robert Paslawski5, Urszula Paslawska5, Lukasz Kownacki6, Jaroslaw Wolinski4, Agnieszka Dobrzyn7, Artur Kaminski8.
1Laboratory, Polbionica Ltd., Warsaw, Poland; 2Laboratory, Foundation of Science Research and Development, Warsaw, Poland; 3Center for Pathomorphological Diagnostics LTD, Warsaw, Poland; 4Institute of Animal Physiology and Nutrition Jan Kielanowski of the Polish Academy of Sciences, Jablonna, Poland; 5Veterinary Institute, Nicolaus Copernicus University, Torun, Poland; 6Radiology, Europejskie Centrum Zdrowia, Warsaw, Poland; 7Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland; 8Medical University of Warsaw, Warsaw, Poland
Introduction: There is lack organs for transplantation. The transplantation of the pancreas is recommended in diabetes with complications. The combination of cell biology and 3D-bioprinting can create organs with vasculature which should be functional. There are some issues to be solved: leakage, thrombosis, enhancement against high pressure, connecting organ to the recipient. The purpose of this study was to 3D-bioprint bionic pancreas which maintain a stable flow in large animals.
Material and methods: 3 pancreas geometries were calculated and bio-printed. The mechanical properties were tested in a bioreactor-20 tests. After bioprinting, the external vessels were attached: decellularized vessels(DV) and vascular prostheses(VP). Studies in animals were carried out on 5 pigs.
Results: Bionic pancreas with internal vessels of 1 mm was bio-printed. Bionic pancreas were stable up to 200 mmHg of pressure in bioreactor. In animals there was a flow throughout the organ after release of vascular clamp. In two cases(DV)-sudden stop of flow within one hour from transplantation with cloths in DV was observed. In next three cases VP were used. In third case stable flow through the organ was observed for 24 hours. Then a thrombotic cloth was observed within an organ. Cloths were in the intersection between printing lines of the internal vessels which were printed transversely to the flow. The g-code and model were changed. Then stable flow was observed with ultrasound and radiographs examination during observed period in following cases.
Conclusion: 3D-bioprinted organ can be successfully fixed to the recipient and maintain flow through the organ.
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